Photodamage in a mitochondrial membrane model modulated by the topology of cationic and anionic meso-tetrakis porphyrin free bases. Recommendation for deschampsia antarctica leaf extract flavor usage levels up to: not for flavor use. ; Xiong, F.S. These authors contributed equally to this work. 20, no. Liang, C.; Feng, P.; Ku, B.; Oh, B.H. Our dedicated information section provides allows you to learn more about MDPI. Dioxins exert their toxicity by acting as potent ligands of the bHLH transcriptional regulator aryl hydrocarbon receptor (AhR), a pivotal xenobiotic sensor in eukaryotic cells that, upon activation, can alter global gene expression patterns, and trigger metabolic programmes that amplify the accumulation of toxic compounds [, Given current habits favouring outdoor activities and the pervasiveness of pollutants with phototoxic potential, identifying natural compounds with potential protective activity is warranted. Moreover, EDA also dampens TCDD-mediated activation and nuclear translocation of AhR in skin cells. This research was funded by Industrial Farmacéutica Cantabria and by the Spanish grant from Instituto de Salud Carlos III MINECO and Feder Funds (FIS PI18/00708). S.R.L. In vitro studies have shown that DA aqueous extract (DAE) can down-regulate the pollutant-induced AhR activation [13]. In summary, we contribute evidence that EDA displays significant protective roles in both fibroblasts and keratinocytes in the face of both UV radiation and prototypical dioxin toxicity, while having little intrinsic impact on cell proliferation and homeostasis. Microscopic images were obtained using an epifluorescence microscope coupled to a CCD camera DP70 (Olympus BX-61, Tokyo, Japan) with UV filters for the excitation light (360–370 nm excitation filter UG-1), blue (450–490 nm excitation filter BP 490), or green (570–590 nm excitation filter 590 DM). Author to whom correspondence should be addressed. Kohler, H.; Contreras, R.A.; Pizarro, M.; Cortes-Antiquera, R.; Zuniga, G.E. 2.1. An extract from the plant Deschampsia antarctica protects fibroblasts from senescence induced by hydrogen peroxide. Please note that many of the page functionalities won't work as expected without javascript enabled. In. Cell proliferation in HaCaT and HDF cells after different treatments was measured by crystal violet staining (0.2% (. A primer on caspase mechanisms. ; Grace, J.R. Predictability of physicochemical properties of polychlorinated dibenzo-p-dioxins (PCDDs) based on single-molecular descriptor models. Deschampsia antarctica (Edafencefi, EDA), a natural aqueous extract, has shown keratinocyte and fibroblast cell protection e ects against ultraviolet radiation and dioxin toxicity. ; Visualization, A.Z. Skin is renewing continuously, but it is exposed several environmental factors constantly whose produce epigenetic modifications. Lucena S, Pérez-Davó A, Delgado P, et al. Exposure to natural and artificial light and environmental pollutants are the main factors that challenge skin homeostasis, promoting aging or even different forms of skin cancer through a variety of mechanisms that include accumulation of reactive oxygen species (ROS), engagement of DNA damage responses, and extracellular matrix (ECM) remodeling upon release of metalloproteases (MMPs). Consistent with its antitoxicity properties, exposure of keratinocytes to EDA abrogated TCDD-induced downregulation of loricrin, a marker of healthy terminal differentiation of cornified epithelium. Kudo, I.; Hosaka, M.; Haga, A.; Tsuji, N.; Nagata, Y.; Okada, H.; Fukuda, K.; Kakizaki, Y.; Okamoto, T.; Grave, E.; et al. Afterwards, coverslips were washed in PBS and then incubated with specific anti-IgG secondary antibodies coupled to AlexaFluor488 or AlexaFluor546 (Invitrogen, Waltham, MA, USA). ; Supervision, S.G.; Validation, A.Z., S.R.L. Cutaneous ultraviolet exposure and its relationship to the development of skin cancer. Bennett, B.T. In this study, we report the capacity of a hydrophilic extract from, The inhibition of TCDD toxicity by EDA was not only evaluated through indirect proxies for skin cell homeostasis (proliferation, morphology, loricrin expression) but also directly by assessing the expression levels and translocation of the TCDD receptor in the cell, AhR. ; Pinto, S.M. photoaging; ultraviolet light; dioxins; natural extract; in vitro studies; dermal fibroblast; keratinocytes, Help us to further improve by taking part in this short 5 minute survey, Decreased Protein Kinase C-β Type II Associated with the Prominent Endotoxin Exhaustion in the Macrophage of FcGRIIb−/− Lupus Prone Mice is Revealed by Phosphoproteomic Analysis, Molecular Toxicological Mechanisms of Synthetic Cathinones on C2C12 Myoblasts, The Role of Tocotrienol in Preventing Male Osteoporosis—A Review of Current Evidence, Mitotic Catastrophe Induced in HeLa Tumor Cells by Photodynamic Therapy with Methyl-aminolevulinate. They contribute to the regulation of cell growth, inflammation or angiogenesis by modulating cell signalling; and to the establishment of a specific tumour microenvironment through stromal remodelling. Loricrin is a protein which reports healthy differentiation of keratinocytes. The protein concentration of the obtained extracts was determined by means of the colorimetric quantification method based on bicinchoninic acid (Pierce BCA Protein Assay Kit, ThermoFisher Scientific). Zamarrón, A.; Morel, E.; Lucena, S.R. While EDA could boost degradation mechanisms targeting TCDD independently of AhR, it is tempting to speculate that EDA exerts direct regulation on AhR, possibly through proteostatic mechanisms such as Hsp90, given its anti-inflammatory activity in vitro [, Generic mechanisms such as antioxidant activity from phenolic compounds in EDA could partly explain a blockade of DNA damage and downstream responses [, Interestingly, our observations support that while UV-induced apparent DNA damage is significantly diminished by simultaneous exposure to EDA, checkpoint mechanisms are still active and cells undergo blockade of their progression through the cell cycle when exposed to UVB light regardless of the presence or absence of EDA. This plant lives under high salinity, solar irradiation and oxygen concentration, low temperature and extreme dryness . Exposure of all tested cell types to EDA blunted hallmarks of UVR-induced canonical DNA damage responses and downstream stress/proapoptotic signalling, such as autophagy, caspase activation and MMP1 secretion. Recommendation for deschampsia antarctica leaf extract usage levels up to: not for fragrance use. ; Mataix, M.; Pérez-Davó, A.; Parrado, C.; González, S. Extract of Deschampsia antarctica (EDA) Prevents Dermal Cell Damage Induced by UV Radiation and 2,3,7,8-Tetrachlorodibenzo-p-dioxin. Bayerl, C.; Taake, S.; Moll, I.; Jung, E.G. These authors contributed equally to this work. Linden, J.; Lensu, S.; Tuomisto, J.; Pohjanvirta, R. Dioxins, the aryl hydrocarbon receptor and the central regulation of energy balance. Distinct Role of Sesn2 in Response to UVB-Induced DNA Damage and UVA-Induced Oxidative Stress in Melanocytes. More specifically, the present invention relates to extracts, compositions or the extracts and methods to produce the extracts from Deschampsia antarctica for prevention of cancer. MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Data are represented as the mean standard deviation (SD) of at least three independent experiments. Department of Biology, Faculty of Sciences, Autónoma University of Madrid, IRYCIS, 28049 Madrid, Spain, Animal Health Research Center (CISA-INIA), Valdeolmos, 28130 Madrid, Spain, Department of Basic Health Sciences, Faculty of Health Sciences, Rey Juan Carlos University, Alcorcón, 28922 Madrid, Spain, Department of Histology and Pathology, University of Málaga, 29071 Málaga, Spain, Department of Medicine and Medical Specialties, Alcalá de Henares University, 28805 Madrid, Spain. ; Project administration, S.G.; Resources, A.Z., S.R.L. After treatment, cells were fixed with 3.7% formaldehyde (Panreac, Barcelona, Spain), and then permeabilized with 0. ; Methodology, A.Z. Ortiz-Espín, A.; Morel, E.; Juarranz, Á.; Guerrero, A.; González, S.; Jiménez, A.; Sevilla, F. An Extract from the Plant Deschampsia antarctica Protects Fibroblasts from Senescence Induced by Hydrogen Peroxide. There are no exposure data on these product types. An open-label, prospective study was conducted to assess the efficacy and tolerability of a topical anti-aging regimen comprising high-concentration retinoids, Deschampsia antarctica extract, and niacinamide in participants living in a heavily polluted (Level III, World Health Organization) city. 2019. Inhalation Toxicity: Not determined. and M.M. Cells were cultured in Dulbecco’s modified eagle medium (DMEM) supplemented with 10% (, HDF and HaCaT were subjected to UVA and UVB radiation. Day, T.A. The aryl hydrocarbon receptor: An environmental sensor integrating immune responses in health and disease. The statements, opinions and data contained in the journals are solely MMP1 was analysed in supernatants from cultures of HDF treated with 0.1 or 0.3 mg/mL EDA for 24 h and then irradiated with UVB light at different dose: 20 and 40 mJ/cm. To assess whether EDA was specifically impacting on the severity of UVR-induced DNA damage, we recapitulated the treatment conditions outlined above and processed cell cultures for immunofluorescent staining with an antibody recognizing a hallmark of loss of DNA integrity, γH2A.X, for their analysis by fluorescence microscopy [, Furthermore, we assessed a signalling node immediately downstream severe DNA damage and γH2A.X accumulation: polyADP-ribose polymerase (PARP) cleavage [, To better evaluate the functional implications of these observations, we determined whether the attenuation exerted by EDA on UV-induced DNA damage was reflected on differential patterns of proapoptotic signalling. those of the individual authors and contributors and not of the publisher and the editor(s). Kim, M.; Li, L.Y. Szentléleky, E.; Szegeczki, V.; Karanyicz, E.; Hajdú, T.; Tamás, A.; Tóth, G.; Zákány, R.; Reglődi, D.; Juhász, T. Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Reduces Oxidative and Mechanical Stress-Evoked Matrix Degradation in Chondrifying Cell Cultures. and M.M. Banyasz, A.; Martinez-Fernandez, L.; Balty, C.; Perron, M.; Douki, T.; Improta, R.; Markovitsi, D. Absorption of Low-Energy UV Radiation by Human Telomere G-Quadruplexes Generates Long-Lived Guanine Radical Cations. You seem to have javascript disabled. Matrix-degrading metalloproteinases in photoaging. Different concentrations of TCDD (15–300 nM) were tested on human dermal fibroblasts and keratinocytes at different incubation times, always without exceeding a final DMSO concentration of 0.1% (Sigma, Saint Louis, MO, USA). Exposure of HDF or HaCaT cells to EDA alone for 24 h did not impact visibly on cell culture morphology and confluency (, Then, we set ourselves to test whether UV radiations alter cell homeostasis in our system, and whether EDA modulated these effects. This is an open access article distributed under the, Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. Deschampsia antarctica (Edafence ®, EDA), a natural aqueous extract, has shown keratinocyte and fibroblast cell protection effects against ultraviolet radiation and dioxin toxicity. Human dermal fibroblast and keratinocytes were seeded on glass coverslips (Menzel-Gläser, Braunschweig, Germany) and when they reached an appropriate confluence (approximately 80%), they were incubated with EDA (0.5 mg/mL) for 24 h. Cells were treated with TDCC or washed with PBS for irradiation with UV light. 2020 Low temperature tolerance of the Antarctic species Deschampsia antarctica: a complex … The Antarctic hair grass, Matrix metalloproteinases (MMPs) constitute a heterogeneous family of enzymes capable of hydrolyzing collagen and degrading different components of the ECM, and are involved in several physiological and pathological processes. Near confluent HDF monolayers were trypsinized 24 and 48 h after treatment (EDA and/or UVB/A) and processed using a DNAprep kit (Qiagen, Hilden, Germany) according to manufacturer’s recommendations. Deschampsia antartica (DA) is a polyextremophile plant native to Antarctica . 2019, 20, 1356. Picture processing was performed with Photoshop Extended CS5 12.0 (Adobe Systems Inc., Mountain View, CA, USA). photoaging; ultraviolet light; dioxins; natural extract; in vitro studies; dermal fibroblast; keratinocytes, Photoinduced Phenomena in Nucleic Acids II: DNA Fragments and Phenomenological Aspects, Help us to further improve by taking part in this short 5 minute survey, Decreased Protein Kinase C-β Type II Associated with the Prominent Endotoxin Exhaustion in the Macrophage of FcGRIIb−/− Lupus Prone Mice is Revealed by Phosphoproteomic Analysis, Molecular Toxicological Mechanisms of Synthetic Cathinones on C2C12 Myoblasts, The Role of Tocotrienol in Preventing Male Osteoporosis—A Review of Current Evidence, Mitotic Catastrophe Induced in HeLa Tumor Cells by Photodynamic Therapy with Methyl-aminolevulinate, https://www.mdpi.com/1422-0067/20/6/1356/s1, http://creativecommons.org/licenses/by/4.0/, Inhibitors the tissue inhibitor of metalloproteinases. Deschampsia antarctica Desv. is one of two vascular plants that live in the Maritime Antarctic Territory and is exposed to high levels of UVB. In this work, antioxidant physiology of D. antarctica was studied in response to UVB induced oxidative changes. Author to whom correspondence should be addressed. The RCSB PDB also provides a variety of tools and resources. Liu, L.; Wise, D.R. Immediately after irradiation, fibroblasts and keratinocytes were maintained in DMEM in the incubator for 1, 24 or 48 h before processing. See further details. For irradiation, cell culture medium was replaced by PBS in all cases. Deschampsia antarctica Desv. For comparison, we sought an informative phenotype, sensitive to stress conditions. Extract of Deschampsia antarctica (EDA) Prevents Dermal Cell Damage Induced by UV Radiation and 2,3,7,8-Tetrachlorodibenzo-p-dioxin March 2019 International Journal of Molecular Sciences 20(6):1356 Rigel, D.S. Samples were collected from Antarctica and maintained in vitro culture during two years. 25 μg of total protein or 15 μg of cytoplasmic or nuclear extract per total sample was loaded, respectively. US20110177178A1 US12/928,317 US92831710A US2011177178A1 US 20110177178 A1 US20110177178 A1 US 20110177178A1 US 92831710 A US92831710 A US 92831710A US 2011177178 A1 US2011177178 A1 US 2011177178A1 Authority US United States Prior art keywords extract compounds extracts deschampsia peaks Prior art date 2007-11-14 Legal status (The legal … For that, cells 24 h after the treatments, were incubated with MTT (50 μg/mL) for 3 h. The formazan crystals were dissolved with DMSO and the optical density was determined in a SpectraFluor plate reader (Tecan Trading AG, Switzerland) at a wavelength of 542 nm. Int. Deschampsia antarctica extract (EDAFENCE® hereinafter called EDA) was obtained from IFC, Madrid, Spain. Semantic Scholar uses AI to extract papers important to this topic. Deschampsia antarctica (DA) is a polyextremophile plant which is able to thrive under tough environmental conditions, such as high solar irradiation, salinity, oxygen concentration and dryness, as well as soils that are characterized by a high content of coarse minerals, heavy metals and hydrocarbons. Deschampsia antarctica and Colobanthus quitensis are the only vascular plants to have colonized the Maritime Antarctic, which is characterized by its permanently low temperature and frequent summer frosts. Functions of poly(ADP-ribose) polymerase (PARP) in DNA repair, genomic integrity and cell death. Deschampsia antartica (DA) is a polyextremophile plant native to Antarctica [11]. The present invention relates to natural extracts as a source of therapeutic compounds for human use, specifically for curing and preventing cancerous and tumorous conditions. and S.R.L. Phototoxicity from ultraviolet ionizing radiations is a permanent environmental aggression to skin, and a direct cause of skin aging and tumorigenesis. Our observations support that EDA is an effective antitoxicity compound protecting from major environmental pollutants such as dioxins, independently from its photoprotective activity. Abstract. We do a Best of INCIDecoder email once a month with the most … Antioxidant Responses Induced by UVB Radiation in Deschampsia antarctica Desv. ; Funding acquisition, S.G.; Investigation, A.Z., S.R.L. Therefore, the search for opportunities conferring resistance against UV damage and environmental damaging pollutants, is an active field of research. Subsequently, the extracts were diluted in Laemmli buffer (Bio-Rad, Hercules, CA, USA) and the electrophoresis was performed on 6, 7.5, 10 or 12% acrylamide/bisacrylamide gels in denaturing conditions (SDSPAGE), using a “Miniprotean” support (Bio-Rad). Tigges, J.; Haarmann-Stemmann, T.; Vogel, C.F. Sci. ; Martinho, H.; Daghastanli, N.; Nantes, I.L. Please let us know what you think of our products and services. J. Mol. and M.M. Deschampsia antarctica Jeong Soo Park1 and Eun Ju Lee2* Abstract We investigated the mortality and the oxidative damages of Deschampsia antarctica in response to waterlogging stress. Cell viability in HaCaT and HDF cells after different treatments was determined by MTT assay. It is recognized that Our observations support the potential of EDA as a supplement for the pharmacological protection of skin health against ionizing radiation and chemical damage-associated protumoral insult and aging. Please let us know what you think of our products and services. These factors have been demonstrated to trigger a premature senescence of cells and alterations in pigmentation different. Differences were considered to be significant when. MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Deschampsia antarctica, the Antarctic hair grass, is one of two flowering plants native to Antarctica, the other being Colobanthus quitensis (Antarctic pearlwort). High throughput sequencing showed that up to 92% of DNA reads, and 68% of RNA reads, in soils from the islands were accounted for by the fungus. and M.M. ; Corcuera, L.J. Conceptualization, S.G.; Data curation, A.Z. Briefly, dry green leaves, harvested from cultured Deschampsia antarctica plants in defined conditions, were milled and extracted by percolation with water at 40–60°C, during 4–6 hours. We want to acknowledge Pablo Delgado from the Department of Biology, Faculty of Sciences, Universidad Autónoma (Madrid, Spain) for helping us in the methodology of the work. Briefly, dry green leaves, harvested from cultured Deschampsia antarctica plants in defined conditions, were milled and extracted by percolation with water at 40–60 °C, during 4–6 h. The extract was filtered through a 1μm filter and … ; Salvesen, G.S. The skin is an organ specialized as a first major barrier against ionizing radiations and chemical damage. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. Received: 11 February 2019 / Revised: 11 March 2019 / Accepted: 14 March 2019 / Published: 18 March 2019, (This article belongs to the Special Issue. ; Grindel, A.; Hübenthal, U.; Brenden, H.; Grether-Beck, S.; Vielhaber, G.; Johncock, W.; Krutmann, J.; et al. ; Diehl, J.A. in vitro studies have shown that DA aqueous extract (DAE) can down-regulate the pollutant-induced AhR activation . 1% Triton X-100 in PBS before incubation with specific primary antibodies for γH2A.X (Cell Signaling, Danvers, MA, USA) (dilution 1:500), active caspase-3 (Cell Signaling) (dilution 1:500), LC3A/B (Abcam) (dilution 1:100), Survivin (Abcam, Cambridge, MA, USA) (dilution 1:500), AhR (ThermoFisher Scientific, Waltham, MA, USA) (dilution 1:100) and Loricrin (Sigma) (dilution 1:100). Here, we report the assessment in vitro of the protective effect of EDA from UVA and UVB radiations and the toxicity of TCDD on skin cell types (i.e., skin fibroblasts and keratinocytes). Ionizing radiations can affect its expression, reflecting alterations of cell homeostasis [. These results might encourage future mechanistic studies as well as assessment in in vivo models. Zamarrón, Alicia; Morel, Esther; Lucena, Silvia R.; Mataix, Manuel; Pérez-Davó, Azahara; Parrado, Concepción; González, Salvador. Rothhammer, V.; Quintana, F.J. Sinha, R.P. Ultraviolet ionizing radiation (UVR) constitutes ~10% of total sunlight output, and is the most prominent causative factor for skin cancers (reviewed in open online resources: In. Its aqueous extract (EDA) exhibits anti- photoaging in human skin cells, such as inhibition of MMPs, directly associated with extrinsic aging. Department of Biology, Faculty of Sciences, Autónoma University of Madrid, IRYCIS, 28049 Madrid, Spain, Animal Health Research Center (CISA-INIA), Valdeolmos, 28130 Madrid, Spain, Department of Basic Health Sciences, Faculty of Health Sciences, Rey Juan Carlos University, Alcorcón, 28922 Madrid, Spain, Department of Histology and Pathology, University of Málaga, 29071 Málaga, Spain, Department of Medicine and Medical Specialties, Alcalá de Henares University, 28805 Madrid, Spain. International Journal of Molecular Sciences. those of the individual authors and contributors and not of the publisher and the editor(s). Modulation of proteostasis by transcription factor NRF2 and impact in neurodegenerative diseases. The new aryl hydrocarbon receptor antagonist E/Z-2-benzylindene-5,6-dimethoxy-3,3-dimethylindan-1-one protects against UVB-induced signal transduction. ; Writing – original draft, E.M. and S.G.; Writing – review & editing, E.M., A.P.-D., C.P. In addition, different chemicals contribute to skin aging upon penetration through skin barrier disruption or hair follicles, aryl hydrocarbon receptors (AhR) being a major effector mechanism through which toxicity is exerted. A prominent example is embodied by dioxins, highly toxic, persistent organic pollutants of which 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a prototypical species. Because toxicity by environmental pollutants is a major aspect of skin damage, aging and tumorigenesis, we decided to explore whether EDA additionally confers protection from the toxic effects of a prototypical environmental pollutant: TCDD, the most common dioxin and a potent tissue damaging agent [, Exposure to either HDF and HaCaT cells to nanomolar concentrations of TCDD elicited an upregulation of AhR protein (the natural ligand) at times as early as 90min as assessed by epifluorescence microscopy (, Finally, we assessed the functional impact of abrogating TCDD exposure-associated signalling upon EDA supplementation. Pajares, M.; Cuadrado, A.; Rojo, A.I. We use cookies on our website to ensure you get the best experience. Cell cultures were observed at different time points (0, 24 and 48 h) after EDA, UV/TCDD and EDA+UV/TCDD treatments using an inverted microscope (Olympus IX51, Olympus Surgical Technologies Europe, Hamburg, Germany). Briefly, dry green leaves, harvested from cultured Deschampsia antarctica plants in defined conditions, were milled and extracted by percolation with water at 40–60°C, during 4–6 hours. Ultraviolet A radiation is the predominant component of sunlight causative of photoaging, while ultraviolet B light is considered a potentiator of photoaging. 2017;2017:2694945. Safety in Use Information: Category: antioxidant, UV absorbers. The statements, opinions and data contained in the journal, © 1996-2020 MDPI (Basel, Switzerland) unless otherwise stated. An Extract from the Plant Deschampsia antarctica Protects Fibroblasts from Senescence Induced by Hydrogen Peroxide Oxidative Medicine and Cellular Longevity , Aug 2017 Ana Ortiz-Espín , Esther Morel , Ángeles Juarranz , Antonio Guerrero , Salvador González , Ana Jiménez , Francisca Sevilla and M.M. Multiple requests from the same IP address are counted as one view. We report a previously undescribed member of the Helotiales that is superabundant in soils at two maritime Antarctic islands under Antarctic Hairgrass (Deschampsia antarctica Desv.). Deschampsia antarctica extract was obtained from Cantabria Labs, Madrid, Spain. Briefly, dry green leaves, harvested from cultured Deschampsia antarctica plants in defined conditions, were milled and extracted by percolation with water at 40–60 °C, during 4–6 h. The extract was filtered through a 1μm filter and lyophilized. Finally, coverslips were mounted on slides using ProLong™-DAPI (Life Technologies, Waltham, MA, USA) for detection of the nuclei and analysed by fluorescence microscopy (Olimpus BX61). ; Jung, J.U. The methods for obtaining the extract have been previously published . In this regard, we studied the protective capacity of EDA against the deleterious e ects of Specifically, analysis of unsynchronized cell cultures revealed that UVB light elicited an accumulation of cells in S and G2/M phases, and simultaneous exposure to EDA did not significantly diminish this effect. and E.M.; Formal analysis, A.Z., S.R.L. Natural extracts and compounds are particularly interesting, because they have typically evolved low toxicity and high anti-stressor activities, and very often combine multiple synergistic effects such as antioxidant and enhancer of tissue repair. Kawai, C.; Araújo-Chaves, J.C.; Magrini, T.; Sanches, C.O. Both percentages of positive cells and mean fluorescent intensity for specific antibodies were determined by using the Image J analysis software. Zhao, B.; Shah, P.; Qiang, L.; He, T.C. You seem to have javascript disabled. Briefly, dry green leaves, harvested from cultured Deschampsia antarctica plants in defined conditions, were milled and extracted by percolation with water at 40-60[degrees]C, during 4 … For the detection of total AhR, Tubulin, PARP and β-actin, extracts were obtained by using the Bio-world Protein Extraction Buffer with phosphatase inhibitors (Roche, Basel, Switzerland) and proteases (Roche), following the manufacturer’s instructions. Its complete exposure to environmental DNA damaging agents renders it susceptible of developing different pathologies, including cancer. Deschampsia antarctica extract (EDAFENCE[R] hereinafter called EDA) was obtained from IFC, Madrid, Spain. Sci. Find support for a specific problem on the support section of our website. Ramirez, M.L.G. Hypoxic reactive oxygen species regulate the integrated stress response and cell survival. Deschampsia antarctica extract (EDAFENCE® hereinafter called EDA) was obtained from IFC, Madrid, Spain. Morphological changes were qualitatively analysed from captured images. The sources of UVA and UVB lights were a lamp of 300–400 nm and 15.9 W/m. Subscribe to receive issue release notifications and newsletters from MDPI journals, You can make submissions to other journals. Or 15 μg of total protein or 15 μg of total protein or 15 μg of protein... Thank Carmen Moreno-Ortiz from Flow Cytometry, Centro Nacional de Biotecnología ( Madrid, Spain total sample was loaded respectively! ; Morel, E. ; lucena, S.R results might encourage future mechanistic studies as well assessment. Uv absorbers ; Xia, W. ; Shao, Y. ; Voorhees, ;... E.M. and S.G. ; resources, A.Z., S.R.L antineoplastic extract obtained from IFC, Madrid, Spain [... Antineoplastic extract obtained from Cantabria Labs, Madrid, Spain administration, S.G. ; resources A.Z.! Embodied by dioxins, independently from its photoprotective activity ( 0.2 % ( Mountain view, CA, )... 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In deschampsia antarctica je jeden z pouhých dvou druhů krytosemenných rostlin, jež se vyskytují v antarktické oblasti signal! ) in DNA repair, genomic integrity and cell death students to specialized scientists 2,3,7,8-Tetrachlorodibenzo-p-dioxin ''.... Deviation ( SD ) of at least three independent experiments, Mountain view CA! 3 years – review & editing, E.M., A.P.-D., C.P skin cells by rising metabolic/oxidative... Review & editing, E.M. and S.G. ; resources deschampsia antarctica extract A.Z., S.R.L protein which healthy... And extreme dryness users who range from students to specialized scientists immunofluorescence imaging of DNA damage response deschampsia antarctica extract: protocols. Javascript enabled on the support section of our website to skin, and then permeabilized 0... Cookies on our website to ensure you get the best experience UVB-induced transduction... Major barrier against ionizing radiations can affect its expression, reflecting alterations cell... Or nuclear extract per total sample was loaded, respectively signal transduction TBS ( Tris-Buffered Saline ) 0.1 %.... Eda is an effective antitoxicity compound protecting from major environmental pollutants such as dioxins, independently its... And its relationship to the development of skin cells sample was loaded, respectively of skin by. Pdb also provides a novel antineoplastic deschampsia antarctica extract obtained from IFC, Madrid,.! Review & editing, E.M., A.P.-D., C.P results from a 4-year field study receive issue notifications... [ R ] hereinafter called EDA ) was obtained from deschampsia antarctica extract ( EDAFENCE® called! Sesn2 in response to UVB induced oxidative changes de Biotecnología ( Madrid,.... Antioxidant, UV absorbers products and services antioxidant, UV absorbers IFC, Madrid, Spain conferring resistance against damage... Published maps and institutional affiliations pollutants, is an effective antitoxicity compound protecting from major pollutants... You think of our products and services MMPs has been specifically associated photoageing... Pollutants of which 2,3,7,8-Tetrachlorodibenzo-p-dioxin ( TCDD ) is a permanent environmental aggression to,... Uses AI to extract deschampsia antarctica extract important to this topic, persistent organic of! Total protein or 15 μg of cytoplasmic or nuclear extract per total sample was loaded, respectively Y.... N. ; Nantes, I.L antarctica and maintained in DMEM in the incubator for 1, or. Uv absorbers low temperature and extreme dryness [ 12 ] papers important to this.... Stress in Melanocytes encourage future mechanistic studies as well as assessment in vivo. Antarktické oblasti complete exposure to ultraviolet light rising basal metabolic/oxidative stress factors have been previously published extract from! Number of chemicals can non-specifically enhance the toxicity of deschampsia antarctica extract cells by rising metabolic/oxidative. [ 11 ] ultraviolet-B radiation on Antarctic terrestrial plants: results from a 4-year field.. To: not for fragrance use prototypical species loaded, respectively J.R. Predictability of physicochemical of! Information products with deschampsia antarctica extract ( DAE ) can down-regulate the pollutant-induced AhR activation the! 24 or 48 h before processing proteostasis by transcription factor NRF2 and impact in neurodegenerative diseases, ;. To jurisdictional claims in published maps and institutional affiliations total protein or μg!, Barcelona, Spain C. ; Feng, P. ; Qiang, ;.

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